Protein Adsorption, Calcium-Binding Ability, and Biocompatibility of Silver Nanoparticle-Loaded Polyvinyl Alcohol (PVA) Hydrogels Using Bone Marrow-Derived Mesenchymal Stem Cells

Several approaches have evolved to facilitate the exploration of hydrogel systems in biomedical research. In this sense, poly(vinyl alcohol) (PVA) has been widely used (HG) fabrication for several therapeutic applications. The biological properties PVA hydrogels (PVA-HGs) are highly dependent on their interaction with protein receptors and extracellular matrix (mainly calcium) deposition, which there is not enough evidence from existing research yet. Thus, first time, functional properties, like mineral interactions, related proliferation mesenchymal stem cells (MSCs) by silver nanoparticle (AgNP)-loaded (AgNPs-PVA-HGs) were investigated present study. UV absorption spectrum TEM microscopic results showed a maximum absorbance synthesized AgNPs at 409 nm, an average particle size 14.5 ± 2.5 respectively. such as calcium-binding adsorption PVA-HG, accelerated incorporating AgNPs; however, swelling HGs reduced AgNPs, might be due masking free groups (hydroxyl PVA) AgNPs. SEM images presence more porous structure HGs. proliferative effect MSCs increased over culture time day 1 7, cell was upregulated pronounced AgNPs-PVA-HG. addition, both did produce any significant cytotoxicity MSCs. histological (bright light H&E staining) fluorescence cytoskeleton fibrillar MSCs, adhered firmly all More bipolar dense structures seen 7 cultures, Interestingly, cultured AgNPs-PVA-HG produced deposition 7. Accordingly, proved biocompatibility suitable system culturing mammalian regenerative tissue